Western Blot…

Western Blot Course Program  

Lecture 1 İntroduction to Western Blotting

·        Principles

·        Data Analysis

·        Troubleshooting

Theoretical
Lecture 2 Introduction to WB equipment Practical
Lecture 3 Sample and Gel preperation Practical
Lecture 4 Loading samples and running the gel

(Electrophoresis)

Practical
Lecture 5 Electro Transfer (Transfer of proteins to membrane) Practical
Lecture 6 Blocking the membrane and Incubation with the primary antibody Practical
Lecture 7 Incubation with Secondary Antibody Practical
Lecture 8 Imaging ( Dark room applications ve Imaging System app.) Practical
Lecture 9 Interpretation of results Theoretical + Practical
Lecture 10 Group discussions and advice Theoretical