Western Blot…
Western Blot Course Program
Lecture 1 | İntroduction to Western Blotting
· Principles · Data Analysis · Troubleshooting |
Theoretical | |
Lecture 2 | Introduction to WB equipment | Practical | |
Lecture 3 | Sample and Gel preperation | Practical | |
Lecture 4 | Loading samples and running the gel
(Electrophoresis) |
Practical | |
Lecture 5 | Electro Transfer (Transfer of proteins to membrane) | Practical | |
Lecture 6 | Blocking the membrane and Incubation with the primary antibody | Practical | |
Lecture 7 | Incubation with Secondary Antibody | Practical | |
Lecture 8 | Imaging ( Dark room applications ve Imaging System app.) | Practical | |
Lecture 9 | Interpretation of results | Theoretical + Practical | |
Lecture 10 | Group discussions and advice | Theoretical |