{"id":145,"date":"2018-01-14T15:34:25","date_gmt":"2018-01-14T12:34:25","guid":{"rendered":"http:\/\/2018.brs.org.tr\/?page_id=145"},"modified":"2018-02-13T08:29:08","modified_gmt":"2018-02-13T05:29:08","slug":"patch-clamp-2","status":"publish","type":"page","link":"https:\/\/2018.brs.org.tr\/index.php\/patch-clamp-2\/","title":{"rendered":"Patch Clamp"},"content":{"rendered":"

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<\/div>\n
The patch clamp technique is an electrophysiological technique that allows to study of single<\/div>\n
or multiple ion channels in cells. The technique can be applied to a wide variety of cells, but<\/div>\n
is especially useful in the study of excitable cells such as neurons, cardiomyocytes, muscle<\/div>\n
fibers, and pancreatic beta cells. The patch clamp technique is a refinement of the voltage<\/div>\n
clamp. Patch-clamp technique was developed by Erwin Neher and Bert Sakmann. This<\/div>\n
discovery made it possible to record the currents of single ion channel molecules for the first<\/div>\n
time, which improved understanding of the involvement of channels in fundamental cell<\/div>\n
processes such as action potentials and nerve activity. Neher and Sakmann received the Nobel<\/div>\n
Prize in Physiology or Medicine in 1991 for this work.<\/div>\n
<\/div>\n

Patch Clamp Course Program<\/strong>\u00a0<\/strong><\/h2>\n\n\n\n\n\n\n\n\n\n\n\n\n
Lecture 1<\/td>\nWhat is Patch Clamp? Introduction to Patch Clamp technique<\/td>\nTheoretical<\/td>\n<\/tr>\n
Lecture 2<\/td>\nPreparing intra and extracellular solutions and ion channel specific agonists and antagonists<\/td>\nPractical<\/td>\n<\/tr>\n
Lecture 3<\/td>\nFabricating pipettes and initiating Patch Clamp recordings<\/td>\nPractical<\/td>\n<\/tr>\n
Lecture 4<\/td>\nPatch Clamp recordings from DRG neurons<\/td>\nPractical<\/td>\n<\/tr>\n
Lecture 5<\/td>\nPatch Clamp recordings from DRG neurons<\/td>\nPractical<\/td>\n<\/tr>\n
Lecture 6<\/td>\nPatch Clamp recordings from DRG neurons<\/td>\nPractical<\/td>\n<\/tr>\n
Lecture 7<\/td>\nPreparing secondary cell cultures and Patch Clamp recordings from Neuroblastoma cells<\/td>\nPractical<\/td>\n<\/tr>\n
Lecture 8<\/td>\nPatch Clamp recordings from cell lines<\/td>\nPractical<\/td>\n<\/tr>\n
Lecture 9<\/td>\nAnalysis of recordings and form to publishable format<\/td>\nPractical<\/td>\n<\/tr>\n
Lecture10<\/td>\nGroup discussions and advice<\/td>\nTheoretical<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

The patch clamp technique is an electrophysiological technique that allows to study of single or multiple ion channels in cells. The technique can be applied to a wide variety of cells, but is especially useful in the study of excitable cells such as neurons, cardiomyocytes, muscle fibers, and pancreatic beta cells. The patch clamp technique is a refinement of the voltage clamp. Patch-clamp technique was developed by Erwin Neher and… <\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-145","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/2018.brs.org.tr\/index.php\/wp-json\/wp\/v2\/pages\/145","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/2018.brs.org.tr\/index.php\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/2018.brs.org.tr\/index.php\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/2018.brs.org.tr\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/2018.brs.org.tr\/index.php\/wp-json\/wp\/v2\/comments?post=145"}],"version-history":[{"count":9,"href":"https:\/\/2018.brs.org.tr\/index.php\/wp-json\/wp\/v2\/pages\/145\/revisions"}],"predecessor-version":[{"id":807,"href":"https:\/\/2018.brs.org.tr\/index.php\/wp-json\/wp\/v2\/pages\/145\/revisions\/807"}],"wp:attachment":[{"href":"https:\/\/2018.brs.org.tr\/index.php\/wp-json\/wp\/v2\/media?parent=145"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}