{"id":136,"date":"2018-01-14T15:33:27","date_gmt":"2018-01-14T12:33:27","guid":{"rendered":"http:\/\/2018.brs.org.tr\/?page_id=136"},"modified":"2018-02-13T14:30:34","modified_gmt":"2018-02-13T11:30:34","slug":"kalsiyum-sinyali","status":"publish","type":"page","link":"https:\/\/2018.brs.org.tr\/index.php\/kalsiyum-sinyali\/","title":{"rendered":"Kalsiyum Sinyali"},"content":{"rendered":"

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H\u00fccre i\u00e7i kalsiyum konsantrasyonundaki y\u00fckselme ([Ca+2]i), n\u00f6rotransmitter sal\u0131n\u0131m\u0131, kas kontraksiyonu ve oosit fertilizasyonu da dahil olmak \u00fczere bir dizi h\u00fccresel olaylar serisini tetiklemektedir (Clapham, 1995; Berridge MJ, 1993). \u0130ntrasell\u00fcler kalsiyum seviyesindeki y\u00fckselme durumu ve bu y\u00fckselmeye kar\u015f\u0131 olu\u015fan tepki, agoniste ve h\u00fccre t\u00fcr\u00fcne ba\u011fl\u0131 olarak farkl\u0131l\u0131k arz etmektedir. Kalsiyuma duyarl\u0131 boya fura-2’nin (Grynkiewicz, 1985) h\u00fccre membran\u0131ndan giri\u015fi, t\u00fcm h\u00fccre s\u00fcspansiyonlar\u0131nda, adherent h\u00fccre pop\u00fclasyonlar\u0131nda, h\u00fccre alt\u0131 d\u00fczeylerindeki [Ca+2]i \u00f6l\u00e7\u00fcm\u00fcnde de\u011fi\u015fikliklere yol a\u00e7t\u0131\u011f\u0131 rapor edilmi\u015ftir (Lambert D., 1992).<\/p>\n

Nano boyutlarda, h\u00fccre alt\u0131 d\u00fczeylerdeki alanlardaki Ca+2 sinyali dalgalar\u0131ndan t\u00fcm h\u00fccre Ca+2 sinyali dalgalar\u0131na kadar de\u011fi\u015fen geni\u015f bir skalada, mikro saniyeler i\u00e7erisinde \u00e7ok say\u0131da Ca+2 sinyali farkl\u0131 h\u00fccre tipleri taraf\u0131ndan kullan\u0131l\u0131r (Berridge, 2006; Bootman ve ark., 2001). Bu h\u00fccresel Ca+2 sinyallerinin karakteristikleri, dokuya \u00f6zg\u00fc Ca+2 ta\u015f\u0131ma sistemlerinin ekspresyonuna ba\u011fl\u0131d\u0131r (Berridge ve ark., 2000). H\u00fccresel Ca+2 sinyalizasyon \u00e7e\u015fitlili\u011fi, her t\u00fcrl\u00fc durumda Ca+2 de\u011fi\u015fimlerini izlemek i\u00e7in kullan\u0131labilecek tek bir teknik olmad\u0131\u011f\u0131 anlam\u0131na gelir. Bununla birlikte, floresan ratiometrik Ca+2 sinyali \u00f6l\u00e7\u00fcm teknikleri, h\u00fccresel Ca+2 sinyali yan\u0131tlar\u0131n\u0131 analiz etmek i\u00e7in \u00e7ok y\u00f6nl\u00fc olarak yayg\u0131n bir bi\u00e7imde kullan\u0131lmaktad\u0131r. Tsien ve arkada\u015flar\u0131 taraf\u0131ndan (Tsien ve ark., 1982) ilk defa a\u00e7\u0131klanmas\u0131ndan bu yana, floresan Ca+2 indikat\u00f6rleri farkl\u0131 deneysel ortamlarda bir\u00e7ok \u00e7e\u015fit h\u00fccre modellerinde Ca+2 sinyallerinin ara\u015ft\u0131r\u0131lmas\u0131nda kullan\u0131lmaktad\u0131r. Uygun florometrik \u00f6l\u00e7\u00fcm cihazlar\u0131 ve uygun indikat\u00f6rler kullanarak, canl\u0131 h\u00fccreler \u00fczerinde h\u00fccre alt\u0131 d\u00fczeyler de dahil, y\u00fcksek h\u0131zda, zaman a\u015f\u0131m\u0131na u\u011framadan Ca+2 sinyallerini izlemek m\u00fcmk\u00fcnd\u00fcr. Bu b\u00f6l\u00fcm h\u00fccre i\u00e7i Ca+2 konsantrasyonunu izlemek i\u00e7in Fura-2AM gibi sentetik floresan indikat\u00f6rlerinin kullan\u0131lmas\u0131 ile Ca+2 sinyallerinin \u00f6l\u00e7\u00fclmesine y\u00f6nelik pratik yakla\u015f\u0131mlar\u0131 a\u00e7\u0131klamaktad\u0131r.<\/p>\n

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Kalsiyum Sinyali Kurs Program\u0131<\/strong><\/h2>\n<\/td>\n

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1. Ders<\/strong><\/td>\nSol\u00fcsyonlar\u0131n haz\u0131rlanmas\u0131 (1xPBS, Kalsiyum buffer, Kalsiyum Free Buffer vb).<\/td>\nTeorik<\/td>\n<\/tr>\n
2. Ders<\/strong><\/td>\nKalsiyum Sinyali Analizleri-1<\/td>\nPratik<\/td>\n<\/tr>\n
3. Ders <\/strong><\/td>\nKalsiyum Sinyali Analizleri-1<\/td>\nPratik<\/td>\n<\/tr>\n
4. Ders<\/strong><\/td>\nKaspaz 3, Kaspaz 9 ve Apoptozis Analizleri<\/td>\nPratik<\/td>\n<\/tr>\n
5. Ders <\/strong><\/td>\nH\u00fccre i\u00e7i ROS ve Mitokondriyal Depolarizasyon Analizleri<\/td>\nPratik<\/td>\n<\/tr>\n
6. Ders<\/strong><\/td>\nKat\u0131l\u0131mc\u0131lara Kalsiyum Sinyali<\/p>\n

Analizlerinin Yapt\u0131r\u0131lmas\u0131-1<\/td>\n

Pratik<\/td>\n<\/tr>\n
7. Ders<\/strong><\/td>\nKat\u0131l\u0131mc\u0131lara Kalsiyum Sinyali<\/p>\n

Analizlerinin Yapt\u0131r\u0131lmas\u0131-2<\/td>\n

Pratik<\/td>\n<\/tr>\n
8. Ders<\/strong><\/td>\nKat\u0131l\u0131mc\u0131lara Apoptozis, Kaspaz-3, -9, H\u00fccre i\u00e7i ROS ve Mitokondriyal Depolarizasyon Analizlerinin Yapt\u0131r\u0131lmas\u0131.<\/td>\nPratik<\/td>\n<\/tr>\n
9. Ders<\/strong><\/td>\nSonu\u00e7lar\u0131n Hesaplanmas\u0131, \u0130statistiksel Analizi ve Makalede Sunulacak Haz\u0131r Hale Getirilmesi-\u015eekillerin \u00c7izilmesi<\/td>\nPratik<\/td>\n<\/tr>\n
10. Ders <\/strong><\/td>\nTart\u0131\u015fma ve Tavsiyeler<\/td>\nTeorik<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"excerpt":{"rendered":"

    H\u00fccre i\u00e7i kalsiyum konsantrasyonundaki y\u00fckselme ([Ca+2]i), n\u00f6rotransmitter sal\u0131n\u0131m\u0131, kas kontraksiyonu ve oosit fertilizasyonu da dahil olmak \u00fczere bir dizi h\u00fccresel olaylar serisini tetiklemektedir (Clapham, 1995; Berridge MJ, 1993). \u0130ntrasell\u00fcler kalsiyum seviyesindeki y\u00fckselme durumu ve bu y\u00fckselmeye kar\u015f\u0131 olu\u015fan tepki, agoniste ve h\u00fccre t\u00fcr\u00fcne ba\u011fl\u0131 olarak farkl\u0131l\u0131k arz etmektedir. Kalsiyuma duyarl\u0131 boya fura-2’nin (Grynkiewicz, 1985) h\u00fccre membran\u0131ndan giri\u015fi, t\u00fcm h\u00fccre s\u00fcspansiyonlar\u0131nda, adherent h\u00fccre pop\u00fclasyonlar\u0131nda, h\u00fccre alt\u0131 d\u00fczeylerindeki [Ca+2]i \u00f6l\u00e7\u00fcm\u00fcnde… <\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-136","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/2018.brs.org.tr\/index.php\/wp-json\/wp\/v2\/pages\/136","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/2018.brs.org.tr\/index.php\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/2018.brs.org.tr\/index.php\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/2018.brs.org.tr\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/2018.brs.org.tr\/index.php\/wp-json\/wp\/v2\/comments?post=136"}],"version-history":[{"count":10,"href":"https:\/\/2018.brs.org.tr\/index.php\/wp-json\/wp\/v2\/pages\/136\/revisions"}],"predecessor-version":[{"id":841,"href":"https:\/\/2018.brs.org.tr\/index.php\/wp-json\/wp\/v2\/pages\/136\/revisions\/841"}],"wp:attachment":[{"href":"https:\/\/2018.brs.org.tr\/index.php\/wp-json\/wp\/v2\/media?parent=136"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}